yes  (Cell Signaling Technology Inc)

Journal: Journal of cell science

Article Title: Immuno-scanning electron microscopy of islet primary cilia.

doi: 10.1242/jcs.262038

Figure Lengend Snippet: Fig. 6. Arl13b labeling in mouse islet cilia. (A–C) Clean Arl13b immunolabeling is achieved using a monoclonal primary antibody (NeuroMab N295B/66, mouse IgG2a, 1:100). (A) Whole cilium. Taken at magnification 32,500×. Scale bar: 1 μm. (B,C) Distal cilium and tip. Taken at magnification 75,000×. Scale bar: 500 nm. (D,E) Another Arl13b antibody (Proteintech 17711-1-AP, rabbit polyclonal, 1:100) produced heavy background labeling on the islet surface, making it difficult to interpret ciliary signals. (D) Whole cilium. Taken at magnification 35,000×. Scale bar: 500 nm. (E) Cilia base. Taken at magnification 100,000×. Scale bar: 200 nm. Images are representative of >20 cilia per Arl13b antibody staining.

Article Snippet: Primary antibodies for cilia immuno-SEM Antibody Antigen Source Host and isotype Dilution Success Figure AcTUB Acetylated alpha tubulin Proteintech 66200-1-Ig Mouse IgG1 1:100 Yes 2–4 GT335 Polyglutamylated tubulin Adipogen AG-20B-0020 Mouse IgG1k 1:400 Yes 4 IFT88 Intraflagellar transport 88 NSJ Bioreagents F41236 Rabbit IgG 1:50 Yes 5 Arl13b ADP ribosylation factor-like protein 13b Neuromab N295B/66 Mouse IgG2a 1:100 Yes 6 Arl13b ADP ribosylation factor-like protein 13b Proteintech 17711-1-AP Rabbit IgG 1:100 No, high background 6 DNAH5 Axonemal dynein heavy chain 5 Sigma HPA037470 Rabbit IgG 1:100 Yes 7 DNAI1 Axonemal dynein intermediate chain 1 Neuromab 75-372 Mouse IgG1 1:100 Yes 7 Jo u rn al o f Ce ll Sc ie n ce to IFT88 immunolabeling on the axonemal surface and reason that the train complexes might have been partially disrupted by the demembranation process.

Techniques: Labeling, Immunolabeling, Produced, Staining